The applied potential to the chip, the approximation equation, and the R2 parameter are shown on each graph. course of development and the results of testing of the graphene-based sensor for detection of protein molecules are also presented. The biosensor was fabricated by the technology previously developed for the gas sensor. The working capacity of the biosensor was tested with an immunochemical system constituted by fluorescein and monoclonal antibodies (mAbs) binding this dye. for for and 0 for is the energy variable, t is the nearest-neighbor hopping energy, is the normalized factor, and the zero energy corresponds to the Dirac point. It was proposed that the adsorbed atom or molecule can be considered as one-electron (one-hole) adparticle, characterized by single orbital and is given in [15,16]). At zero temperature the occupation number of adparticles quasilevel is given by the sum of the band contribution and local state contribution is the Fermi level and the energy of local state is the root of the equation for [15]. If initially (before adsorption) level was occupied, then the adparticle charge is is the wave-vector Eicosadienoic acid separation from the Dirac point vector [18]. Such a dispersion gives the density of states for and 0 for is the simplified version of the from [15,16]. General expression for the epigraphene DOS is given by is the energy of the graphene-substrate interaction and is the substrate DOS. In what follows, we will consider SiC as a substrate and use the HaldaneCAnderson model for and 0 for is the center of energy gap position relative to the Dirac point. This DOS corresponds to the shift function values for the different SiC polytypes are given in [19]. There are two limiting regimes for the grapheneCsubstrate interaction: Strong coupling, when tends to the DOS of adsorbed single carbon adatom, while in the second case (quasi-free-standing graphene). More rigorous expressions for in both regimes are given in [17]. It is clear that only the second case is of practical interest. Thus, below we will consider only weak coupling regime. It is easy to understand now that for the DOS of particle adsorbed on epigraphene, formula (1) has to be rewritten in the form is the adparticleCepigraphene interaction. Then the occupation number of adparticles quasilevel is is the surface concentration of adparticles and is their concentration in monolayer, one has to include Eicosadienoic acid adparticles interactions in overlayer. The most important is the dipoleCdipole repulsion, which can be taken into account by the replacement of to is the elementary charge, is the adparticle bond length, and [21]. It is worthy to note that all the interactions of adsorbed particles lead to the decrease of is discussed in [22]. The effect of adsorption on the substrate appears in mainly two effects. One is the change in the work function due to the charge transfer between an adparticle and the substrate. As a result of this transfer, the adparticle acquires charge or prevent the escape of an Eicosadienoic acid electron from the substrate, thus, lowering or raising the work function. In the former case, the electron passes from donor adparticle to the substrate; in the latter, it leaves the substrate for acceptor adparticle. The second effect due to adsorption is the change in the surface conductivity of the substrate changes as follows: The donor (acceptor) adparticles increase (decrease) the conductivity of the of the carriers. The systematic studies of the simultaneous changes in the surface conductivity and work function began with experimental works on gas molecules adsorption on metal oxides [23,24]. A theory that relates quantities and was developed in [25,26], where the following equation was obtained: does not explicitly depend on the coverage and have to be HES1 measured simultaneously. In [27], we have applied Equation (3) to the analysis of experimental data on gas molecules adsorption on carbon nanostructures (see corresponding references in [14]). This analysis demonstrates a number of inconsistences of published experimental results with Equation (3). Some additional theoretical estimates are given in Appendix A. 3. Graphene Film Production Technology Interest in graphene flared up after the publication of K.S. Novoselova, A.K. Geim et al., In which they demonstrated the possibility of obtaining graphene sheets using micromechanical cleavage of bulk crystalline graphite [28]. This is because of the exclusive physical and mechanised properties of graphene mainly, such as for example high thermal and electric conductivity, high flexibility of charge companies, high Youngs modulus, mix of optical transparency with great electric conductivity, etc. The listed properties have become attractive from the real perspective of possible applications of.